Research Projects Completed:

• Development of Reproductive Technologies- IVF-ET, GIFT,ZIFT,ICSI,PGD.
• Established for the first time in India the technique of In Vitro Fertilization and Embryo Transfer as a Collaborative efforts between King Edwards Memorial Hospital and Institute of Research in Reproduction (ICMR).
• These efforts resulted in a birth of India's first scientifically documented IVF baby (Test-tube baby) on 6th August 1986.
• Gamete Intrafallopian Transfer (GIFT) technique was developed successfully and India's GIFT baby was born on 4th January 1988.
• Oocyte donation programme in cases of premature ovarian failure. First baby was born on 24th Janaury, 1991.
• Cytogenetic study in couple having unexplained infertility, male infertility-Bad obstetric history.
• Human chorionic villous sampling in high risk pregnancy with history of genetic disorders in past or family history of congenital anomalies.
• Study of chromosomal anomalies in couples belonging to cases having H/O repeated fetal losses or susceptible to fetal loss. Cytogenital study was supported by Research Society of KEM Hospital.
• Induction of Ovulation.
• Administration of Clomiphene Citrates, human menopausal gonadotropin, pure follicular stimulation hormone and human chorionic gonadotropins in different combination and dose regime.
• Effect of oral contraceptives prior to ovarian stimulation its effect on follicular response to stimulation protocol.
• Influence of Gonadotropin Releasing Hormone on stimulation of multiple Follicles.
• Varying time interval of gonadotropin administration and its effect on oocyte maturation and fertilization in vitro.
• Human Oocyte.
• Ultrastructure of immature, mature, atretic, normally fertilized and abnormally fertilized oocyte.
• Correlation between steroid hormones and protein contents of follicular fluid and maturation of oocyte.
• Protein synthesis by granulosa cells surrounding oocyte and fertilizing ability of oocyte.
• Constituents of oocyte environment, study of the follicular fluid for the presence of gonadotropin, steroid hormones, glycosaminoglycans, total protein and glucose.
• Inhibin in follicular fluid and its correlation with maturation, fertilizing ability of oocyte.
• Intraovarian factors contributing follicular maturation, fertilization and atresia.
• Effect of injection of purified human follicular fluid on mice follicles.
• Male Infertility
• Ultrastructure of sperm in infertile male
• Decondensation of sperm in vitro in infertile male
• Study of fertilising potential of sperm. Hypoosmotic test, acrydine orange test, zona free hamster egg penetration with human capacited sperms.
• Cytogenetic Study of Oligospermia
• Unexplained Infertility- Immunological factors responsible of infertility by reducing fertilization potential of gametes.
• Intra Uterine Insemination of washed spermatozoa and its outcome in terms of pregnancy.
• Early Pregnancy Factor.
• Presence of early pregnancy factor in culture fluid and serum as a marker for early embryo implantation.
• Early diagnosis and prognosis of choriocarcinoma and vesicular mole by estimating early pregnancy factor in serum.
• Vitamin Carrier Protein.
• Riboflavin-carrier protein (RCP) in follicular fluid and its correlation with oocyte maturation.
• Presence RCP in fallopian tube in different phases of menstrual cycle.
• Testing of efficacy of ZK 98.299 as a synthetic progesterone antagonist.
• Micromanipulation of Gametes.
• Microfertilization by injecting a sperm in perivitalline space of oocyte ooplasm.
• Intracytoplasmic sperm injection.
• Microbiopsy of mice embryo.
• Prevention of sex linked diseases.
• Inhibin in endometrium during various phases of the ovarian cycle.
• Hypertrophy of Pituitary during different phases of menstrual cycle.
• Pituitary size and its correlation with follicular size and levels of gonadotropins and steroid hormones.
• Molecular studies on defects in human sperm. Jaslok Hospital & Research Centre.
• Genetic evaluation of male infertility (Y deletion). Jaslok Hospital & Research Centre
• Ongoing Research Projects:
• Microdeletion of Y chromosomes in males (collaboration with TIFR)
• Sperm function tests and fertilization in vitro.
• Role of Centrosome in fertilizing potential of the sperm. (collaboration with IRR, ICMR).
• Receptive genes in endometrium useful for implantation. (collaboration with IRR, ICMR).
• To establish human embryonic stem (HES) cell lines without mouse embryonic fibroblast feeder layer and to identify factors secreted by MEF layer that prevents differentiation of HES cells in-vitro. National Institute for Research and Reproduction.
• Purification and characterization of human sperm adhesion molecular identified by polyclonal /monoclonal antibodies and their role in infertility.
• Factors regulating endometrial receptivity in nonhuman primate

List of Publications:

• Hinduja, I. N., Asgaokar V.S. (1969) Single dose of Chloramphenicol in treatment of Enteric Fever. Current Medical Journal., 4, 102-105.
• Hinduja, I. N., Shah S.K. (1969) Ovarian Tomours. Current Medical Journal., 4, 311-314.
• Hinduja, I. N., Thakur S.S. (1971) Septic Abortion (Criminal Abortions). Indian Journal of Obstet. and Gynaecol., 21, 251.
• Hinduja, I. N., Joglekar, S.J. (1974) The comparative study of Coagulation Mechanism in Mid-trimenster Abortions Induced by Prostaglandins and Hypertonic Saline. Indian Journal of Obstet. & Gynaec. 24, 432.
• Hinduja, I. N., Mehta, A.P. (1976) Anaerobic Infections in Obstetrics and Gynaecology. Proceeding of the Third All India Congress of Medical Microbiologists, pp. 323.